fluorescein conjugated uea 1 lectin  (Vector Laboratories)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Inhibition of Galectin-3 Alleviates Cigarette Smoke Extract-Induced Autophagy and Dysfunction in Endothelial Progenitor Cells

doi: 10.1155/2019/7252943

Figure Lengend Snippet: Characterization of cultured human EPCs. PBMC-derived EPCs with a spindle shape formed clones (a) (200x). EPCs were shown to simultaneously endocytose DiI-ac-LDL (red) and bind fluorescein isothiocyanate UEA-1 (lectin, green), and a merged photo was also presented (b) (200x). FACS analysis showed high expression of CD133, CD34, and KDR on EPCs (c). Scale bar = 50 μ m.

Article Snippet: After 14 days of culture, the cells were incubated with fluorescein isothiocyanate-conjugated lectin from Ulex europaeus agglutinin 1 (FITC-UEA-1) (Sigma, Germany), and 1,19-dioctadecyl-3,3,3939-tetramethylindocar-bocyanine perchlorate-labeled acetylated low-density lipoprotein (LDL-ac-Dil) (Sigma, Germany).

Techniques: Cell Culture, Derivative Assay, Clone Assay, Expressing

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Regulation of Gastric Lgr5+ve Cell Homeostasis by Bone Morphogenetic Protein (BMP) Signaling and Inflammatory Stimuli

doi: 10.1016/j.jcmgh.2018.01.007

Figure Lengend Snippet: Lineage tracing of cells with Lgr5 transcriptional activity in H felis –infected Lgr5-Cre;H + /K + -Nog;Rosa26-Tom mice. 1-2-month old Lgr5-Cre;H + /K + -Nog;Rosa26-tdTom mice were treated with one i.p. injection of tamoxifen (0.1 mg/g body weight) and inoculated with H. felis two months post tamoxifen. Animals were analyzed 3 months after inoculation, and 5 months after tamoxifen injection. Gastric paraffin sections of the lesser curvature of H felis –infected Lgr5-Cre;H + /K + -Nog;Rosa26-tdTom mice were stained with anti-tomato (red fluorescent protein) primary antibodies and Alexa 555–conjugated secondary antibodies together with anti-IF antibodies and Alexa 488–conjugated secondary antibodies (IF/Tom), Alexa 488–conjugated GSII (GSII/Tom), Alexa 488–conjugated Ulex europaeus agglutinin 1 (UEA1), anti-CD44 and anti-CD44v9 primary antibodies and Alexa 488–conjugated secondary antibodies (CD44/Tom and CD44v9/Tom), and anti-BrdU primary antibodies and Alexa 488–conjugated secondary antibodies (BrdU/Tom). Scale bars : 50 μm. Similar results were observed in at least 5 other H felis –infected Lgr5-Cre;H + /K + -Nog;Rosa26-tdTom mice except for the experiments with the anti-CD44v9 antibodies that were repeated in 1 other mouse.

Article Snippet: In some experiments the slides were stained for 1.5 hours at 37°C with Alexa 488–conjugated GSII (1:1000, catalog number: FL-1211; Vector Laboratories, Burlingame, CA) and fluorescein isothiocyanate–conjugated Ulex europaeus agglutinin 1 lectin (1:200, catalog number: FL-1061; Vector Laboratories).

Techniques: Activity Assay, Infection, Injection, Staining

Journal: Molecular Medicine Reports

Article Title: Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer

doi: 10.3892/mmr.2018.8623

Figure Lengend Snippet: Characterization of EPCs using fluorescence microscopy. (A) FITC-UEA-1 positive cells identified by fluorescence staining with FITC-UEA-1. (B) Dil-Ac-LDL stained cells. (C) Nuclei counterstained with DAPI (D) Merged panels to reveal double-positive staining with Dil-Ac-LDL and FITC-UEA-1. EPCs, endothelial progenitor cells; FITC-UEA-1, fluorescein isothiocyanate-conjugated Ulex europaeus lectin; Dil-Ac-LDL, 1,1-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein.

Article Snippet: Subsequently, cells were fixed by 4% polyoxymethylene at room temperature for 20 min and counterstained with fluorescein isothiocyanate-conjugated Ulex europaeus lectin (FITC-UEA-1; 10 µg/ml; cat. no. L9006; Sigma-Aldrich; Merck KGaA) for 1 h at room temperature.

Techniques: Fluorescence, Microscopy, Staining, Labeling

Journal: Molecular Medicine Reports

Article Title: Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer

doi: 10.3892/mmr.2018.8623

Figure Lengend Snippet: Determination of the expression of EPC surface markers via flow-cytometry. (A) CD31 and FLK-1 expression, and (B) CD34 and CD133 expression. EPCs, endothelial progenitor cells; FITC, fluorescein isothiocyanate; PE, phycoerythrin; FLK-1, fetal liver kinase-1; CD, cluster of differentiation.

Article Snippet: Subsequently, cells were fixed by 4% polyoxymethylene at room temperature for 20 min and counterstained with fluorescein isothiocyanate-conjugated Ulex europaeus lectin (FITC-UEA-1; 10 µg/ml; cat. no. L9006; Sigma-Aldrich; Merck KGaA) for 1 h at room temperature.

Techniques: Expressing, Flow Cytometry